In the beginning

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We start with a number of very large screenshots (don't be daunted they are just here to orient you). They show the GFtbox graphical interface.

First view

The GFtbox user interface. Focus on the Select Tool Panel. Here the Mesh Editor has been selected and the Mesh Editor panel is visible. Idea: change all GFtbox font sizes using Menu Misc:Gui Format

Add a mesh (canvas that represents tissue)

Create a canvas by clicking on Panel Mesh Editor:new. A variety of preset shapes are available, here we choose a Circle with 4 concentric rings of nodes that is 2 mm in diameter. Idea: Special shapes can be imported as OBJ files.

Add a pattern of growth factor

Select Tool:Factors makes the Morphogen Panel visible. Currently we are controlling the KAPAR factor. It has been selected from a drop down list top right (Plot current factor). We add a radially increasing gradient of KAPAR. Make sure that the Panel Plot Options:Monochrome is ticked and the gradient of KAPAR will be as shown. What will be the result of growth on just the bottom side of the canvas?

And grow

Now save the project using the Save As button. A dialogue box will open - here we keyed in in_the_beginning and the prefix GPT_ and the suffix _date was added automatically. A directory is created with the project name which contains all the files needed for the project and any results (snapshots, movies) we create. Select Tool:Simulation makes the Simulation Panel visible. Change the Panel Run:Run until box to 2 and click the Panel Run:Run until button. The canvas will grow in steps set by the Panel Simulation:Timescale box. Warning: saving is essential if you want to Restart the simulation. Idea: to create an uncompressed movie of your simulation, before running click the Record movie and when it is finished click Stop movie. The movie will be in the Project directory. We normally compress the movies using Microsoft Live Movie Maker or Quicktime.