Imaging Platform: VisiTech QLC100 spinning disc confocal
Software: MetaMorph ver6.2r6 [.stk .tif]

Date:25-11-2010
Time:15:30

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XYZ Image:

x-1344 pixels  [0.330472 um]
y-1024 pixels  [0.330472 um]
z-31 slices  [ 1.00um]
t-01 intervals  [**** sec]
ch-01    [Ex488 > Em500-550nm]


Objective x20/0.5 Plan Fluor (Nikon)
Zoom x1

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XYZC Image:

x-1344 pixels  [0.330472 um]
y-1024 pixels  [0.330472 um]
z-31 slices  [ 1.00um]
t-01 intervals  [**** sec]
ch-01    [Ex488 > Em500-550nm]
         [Ex561 > Em***-***nm] 617/73


Objective x20/0.5 Plan Fluor (Nikon)
Zoom x1

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XYZCT Image:

xx-1344 pixels  [0.330472 um]
y-1024 pixels  [0.330472 um]
z-31 slices  [ 1.00um]
t-03 intervals  [60 sec]
ch-01    [Ex488 > Em500-550nm]
         [Ex561 > Em***-***nm] 617/73


Objective x20/0.5 Plan Fluor (Nikon)
Zoom x1

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XYZ Image 2:

x-1344 pixels  [0.162768 um]
y-1024 pixels  [0.162768 um]
z-61 slices  [ 0.50um]
t-01 intervals  [**** sec]
ch-01    [Ex488 > Em500-550nm]


Objective x40/1.3 oil Plan Fluor (Nikon)
Zoom x1

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Scale bar 100um XY Image:


x-1344 pixels  [0.330472 um]
y-1024 pixels  [0.330472 um]
z-31 slices  [ *****um]
t-01 intervals  [**** sec]
ch-01    [Bright Field]


Objective x20/0.5 Plan Fluor (Nikon)
Zoom x1