Leica Microsystems Heidelberg GmbH This file is intended for read-only purposes changes here will not affect the images. Date: Thursday, November 25, 2010 Time: 17:38 File Version: 26000000 EXPERIMENT INFORMATION Number of Images: 5 Type: Series with 'tif'-files DIMENSION DESCRIPTION #0 Pixel Size in Byte: 1 Resolution in Bit: 8 Max Value: 255.0000000000 Min Value: 0.000000e+000 Label: I Number of Dimensions: 4 Dimension_0: 120 Logical Size: 512 Physical Length: 7.500000e-004 m Physical Origin: 0.000000e+000 m Dimension_1: 121 Logical Size: 512 Physical Length: 7.500000e-004 m Physical Origin: 0.000000e+000 m Dimension_2: 6815843 Logical Size: 1 Physical Length: 0.000000e+000 Physical Origin: 0.000000e+000 Dimension_3: 122 Logical Size: 22 Physical Length: -2.307880e-005 m Physical Origin: -3.398730e-006 m Series Name: XYZ image Description: HARDWARE PARAMETER #0 AOTF (405) 0.000000 AOTF (405) 0.000000 AOTF (458) 0.000000 AOTF (476) 0.000000 AOTF (488) 70.671551 AOTF (514) 0.000000 AOTF (543) 0.000000 AOTF (633) 0.000000 AOTF (458) 0.000000 AOTF (476) 0.000000 AOTF (488) 0.000000 AOTF (514) 0.000000 AOTF (543) 0.000000 AOTF (633) 0.000000 PMT 1 Active Active PMT 1 (Offs.) 3.600000 PMT 1 (HV) 483.333333 PMT 2 Inactive Inactive PMT 3 Inactive Inactive PMT Trans Inactive Inactive Beam Expander Beam Exp 6 Beam Exp 6 Excitation Beam Splitter FW RSP 500 RSP 500 UV Lens FW Lens 10x Lens 10x Hardware Type No. 2.000000 Scan Field Rotation -0.038943 Rotation Direction 1 X Scan Actuator Active Active X Scan Actuator (Gain) 1.000000 X Scan Actuator (Offs.) 0.000000 Y Scan Actuator Active Active Y Scan Actuator (Gain) 1.000000 Y Scan Actuator (Offs.) 0.000000 Z Scan Actuator Inactive Inactive Z Scan Actuator (POS) -0.000014 Scan Speed 400.000000 Phase 20.605469 Y-Phase 0.122100 SP Mirror 1 (left) 505.000000 SP Mirror 1 (right) 555.000000 SP Mirror 1 (stain) SP Mirror 2 (left) 575.833333 SP Mirror 2 (right) 654.166667 SP Mirror 2 (stain) SP Mirror 3 (left) 732.500000 SP Mirror 3 (right) 810.833333 SP Mirror 3 (stain) Objective HC PL APO 20.0x0.70 UV HC PL APO 20.0x0.70 UV Order number (Obj.) 506513 Numerical aperture (Obj.) 0.700000 SCANNER INFORMATION #0 RoiScan 0 IsSequential 0 ChaserUVShutter 0 ChaserVisibleShutter 0 MPShutter 0 UVShutter 0 VisibleShutter 1 ScanMode xyz Active Pinhole [m] 0.000073 Pinhole [airy] 1.001570 Size-Width [µm] 750.000000 Size-Height [µm] 750.000000 Size-Depth [µm] -23.078802 StepSize 0.000001 Voxel-Width [µm] 1.464844 Voxel-Height [µm] 1.464844 Voxel-Depth [µm] 1.098991 Zoom 1.000000 Scan-Direction 1 Y-Scan-Direction 1 SequentialMode 0 Frame-Accumulation 1 Frame-Average 1 Line-Average 4 Resolution 8 Channels 1 Format-Width 512 Format-Height 512 Sections 22 TIME INFORMATION #0 Stamped Dimension: 2 Stamp_0: 2010:11:25,16:58:25:848 Stamp_1: 2010:11:25,16:58:32:348 Stamp_2: 2010:11:25,16:58:38:864 Stamp_3: 2010:11:25,16:58:45:379 Stamp_4: 2010:11:25,16:58:51:879 Stamp_5: 2010:11:25,16:58:58:395 Stamp_6: 2010:11:25,16:59:04:895 Stamp_7: 2010:11:25,16:59:11:410 Stamp_8: 2010:11:25,16:59:17:926 Stamp_9: 2010:11:25,16:59:24:426 Stamp_10: 2010:11:25,16:59:30:941 Stamp_11: 2010:11:25,16:59:37:457 Stamp_12: 2010:11:25,16:59:43:957 Stamp_13: 2010:11:25,16:59:50:473 Stamp_14: 2010:11:25,16:59:56:973 Stamp_15: 2010:11:25,17:00:03:488 Stamp_16: 2010:11:25,17:00:10:04 Stamp_17: 2010:11:25,17:00:16:504 Stamp_18: 2010:11:25,17:00:23:19 Stamp_19: 2010:11:25,17:00:29:535 Stamp_20: 2010:11:25,17:00:36:35 Stamp_21: 2010:11:25,17:00:42:550 LUT DESCRIPTION #0 LUT_0 Name: Green Inverted (1=yes / 0=no): 0 SEQUENTIAL INFORMATION #0 Sequence Count: 0 SERIES INFORMATION #0 Number of Series: 5 IMAGES INFORMATION #0 Number of Images: 22 Image Width: 512 Iamge Length: 512 Bits per Sample: 8 Samples per Pixel: 1 *************************************** NEXT IMAGE ********************************* DIMENSION DESCRIPTION #1 Pixel Size in Byte: 1 Resolution in Bit: 8 Max Value: 255.0000000000 Min Value: 0.000000e+000 Label: I Number of Dimensions: 4 Dimension_0: 120 Logical Size: 512 Physical Length: 7.500000e-004 m Physical Origin: 0.000000e+000 m Dimension_1: 121 Logical Size: 512 Physical Length: 7.500000e-004 m Physical Origin: 0.000000e+000 m Dimension_2: 6815843 Logical Size: 2 Physical Length: 0.000000e+000 Physical Origin: 0.000000e+000 Dimension_3: 122 Logical Size: 22 Physical Length: -2.307880e-005 m Physical Origin: -3.398730e-006 m Series Name: XYZC image Description: HARDWARE PARAMETER #1 AOTF (405) 0.000000 AOTF (405) 0.000000 AOTF (458) 0.000000 AOTF (476) 0.000000 AOTF (488) 70.671551 AOTF (514) 0.000000 AOTF (543) 68.009768 AOTF (633) 0.000000 AOTF (458) 0.000000 AOTF (476) 0.000000 AOTF (488) 0.000000 AOTF (514) 0.000000 AOTF (543) 0.000000 AOTF (633) 0.000000 PMT 1 Active Active PMT 1 (Offs.) 3.600000 PMT 1 (HV) 483.333333 PMT 2 Active Active PMT 2 (Offs.) 3.300000 PMT 2 (HV) 583.308913 PMT 3 Inactive Inactive PMT Trans Inactive Inactive Beam Expander Beam Exp 6 Beam Exp 6 Excitation Beam Splitter FW RSP 500 RSP 500 UV Lens FW Lens 10x Lens 10x Hardware Type No. 2.000000 Scan Field Rotation -0.038943 Rotation Direction 1 X Scan Actuator Active Active X Scan Actuator (Gain) 1.000000 X Scan Actuator (Offs.) 0.000000 Y Scan Actuator Active Active Y Scan Actuator (Gain) 1.000000 Y Scan Actuator (Offs.) 0.000000 Z Scan Actuator Inactive Inactive Z Scan Actuator (POS) -0.000014 Scan Speed 400.000000 Phase 20.605469 Y-Phase 0.122100 SP Mirror 1 (left) 505.000000 SP Mirror 1 (right) 535.000000 SP Mirror 1 (stain) SP Mirror 2 (left) 600.000000 SP Mirror 2 (right) 700.000000 SP Mirror 2 (stain) SP Mirror 3 (left) 732.500000 SP Mirror 3 (right) 810.833333 SP Mirror 3 (stain) Objective HC PL APO 20.0x0.70 UV HC PL APO 20.0x0.70 UV Order number (Obj.) 506513 Numerical aperture (Obj.) 0.700000 SCANNER INFORMATION #1 RoiScan 0 IsSequential 0 ChaserUVShutter 0 ChaserVisibleShutter 0 MPShutter 0 UVShutter 0 VisibleShutter 1 ScanMode xyz Active Pinhole [m] 0.000073 Pinhole [airy] 1.001570 Size-Width [µm] 750.000000 Size-Height [µm] 750.000000 Size-Depth [µm] -23.078802 StepSize 0.000001 Voxel-Width [µm] 1.464844 Voxel-Height [µm] 1.464844 Voxel-Depth [µm] 1.098991 Zoom 1.000000 Scan-Direction 1 Y-Scan-Direction 1 SequentialMode 0 Frame-Accumulation 1 Frame-Average 1 Line-Average 4 Resolution 8 Channels 2 Format-Width 512 Format-Height 512 Sections 22 TIME INFORMATION #1 Stamped Dimension: 2 Stamp_0: 2010:11:25,17:02:29:752 Stamp_1: 2010:11:25,17:02:29:752 Stamp_2: 2010:11:25,17:02:36:252 Stamp_3: 2010:11:25,17:02:36:252 Stamp_4: 2010:11:25,17:02:42:767 Stamp_5: 2010:11:25,17:02:42:767 Stamp_6: 2010:11:25,17:02:49:267 Stamp_7: 2010:11:25,17:02:49:267 Stamp_8: 2010:11:25,17:02:55:782 Stamp_9: 2010:11:25,17:02:55:782 Stamp_10: 2010:11:25,17:03:02:282 Stamp_11: 2010:11:25,17:03:02:282 Stamp_12: 2010:11:25,17:03:08:797 Stamp_13: 2010:11:25,17:03:08:797 Stamp_14: 2010:11:25,17:03:15:313 Stamp_15: 2010:11:25,17:03:15:313 Stamp_16: 2010:11:25,17:03:21:813 Stamp_17: 2010:11:25,17:03:21:813 Stamp_18: 2010:11:25,17:03:28:328 Stamp_19: 2010:11:25,17:03:28:328 Stamp_20: 2010:11:25,17:03:34:843 Stamp_21: 2010:11:25,17:03:34:843 Stamp_22: 2010:11:25,17:03:41:343 Stamp_23: 2010:11:25,17:03:41:343 Stamp_24: 2010:11:25,17:03:47:859 Stamp_25: 2010:11:25,17:03:47:859 Stamp_26: 2010:11:25,17:03:54:358 Stamp_27: 2010:11:25,17:03:54:358 Stamp_28: 2010:11:25,17:04:00:874 Stamp_29: 2010:11:25,17:04:00:874 Stamp_30: 2010:11:25,17:04:07:389 Stamp_31: 2010:11:25,17:04:07:389 Stamp_32: 2010:11:25,17:04:13:889 Stamp_33: 2010:11:25,17:04:13:889 Stamp_34: 2010:11:25,17:04:20:404 Stamp_35: 2010:11:25,17:04:20:404 Stamp_36: 2010:11:25,17:04:26:920 Stamp_37: 2010:11:25,17:04:26:920 Stamp_38: 2010:11:25,17:04:33:420 Stamp_39: 2010:11:25,17:04:33:420 Stamp_40: 2010:11:25,17:04:39:935 Stamp_41: 2010:11:25,17:04:39:935 Stamp_42: 2010:11:25,17:04:46:435 Stamp_43: 2010:11:25,17:04:46:435 LUT DESCRIPTION #1 LUT_0 Name: Green Inverted (1=yes / 0=no): 0 LUT_1 Name: Red Inverted (1=yes / 0=no): 0 SEQUENTIAL INFORMATION #1 Sequence Count: 0 IMAGES INFORMATION #1 Number of Images: 44 Image Width: 512 Iamge Length: 512 Bits per Sample: 8 Samples per Pixel: 1 *************************************** NEXT IMAGE ********************************* DIMENSION DESCRIPTION #2 Pixel Size in Byte: 1 Resolution in Bit: 8 Max Value: 255.0000000000 Min Value: 0.000000e+000 Label: I Number of Dimensions: 5 Dimension_0: 120 Logical Size: 512 Physical Length: 7.500000e-004 m Physical Origin: 0.000000e+000 m Dimension_1: 121 Logical Size: 512 Physical Length: 7.500000e-004 m Physical Origin: 0.000000e+000 m Dimension_2: 6815843 Logical Size: 2 Physical Length: 0.000000e+000 Physical Origin: 0.000000e+000 Dimension_3: 122 Logical Size: 22 Physical Length: -2.307880e-005 m Physical Origin: -3.398730e-006 m Dimension_4: 116 Logical Size: 3 Physical Length: 360.00 s Physical Origin: 0.000000e+000 s Series Name: XYZCT image Description: HARDWARE PARAMETER #2 AOTF (405) 0.000000 AOTF (405) 0.000000 AOTF (458) 0.000000 AOTF (476) 0.000000 AOTF (488) 70.671551 AOTF (514) 0.000000 AOTF (543) 68.009768 AOTF (633) 0.000000 AOTF (458) 0.000000 AOTF (476) 0.000000 AOTF (488) 0.000000 AOTF (514) 0.000000 AOTF (543) 0.000000 AOTF (633) 0.000000 PMT 1 Active Active PMT 1 (Offs.) 3.600000 PMT 1 (HV) 483.333333 PMT 2 Active Active PMT 2 (Offs.) 3.300000 PMT 2 (HV) 583.308913 PMT 3 Inactive Inactive PMT Trans Inactive Inactive Beam Expander Beam Exp 6 Beam Exp 6 Excitation Beam Splitter FW RSP 500 RSP 500 UV Lens FW Lens 10x Lens 10x Hardware Type No. 2.000000 Scan Field Rotation -0.038943 Rotation Direction 1 X Scan Actuator Active Active X Scan Actuator (Gain) 1.000000 X Scan Actuator (Offs.) 0.000000 Y Scan Actuator Active Active Y Scan Actuator (Gain) 1.000000 Y Scan Actuator (Offs.) 0.000000 Z Scan Actuator Inactive Inactive Z Scan Actuator (POS) -0.000014 Scan Speed 400.000000 Phase 20.605469 Y-Phase 0.122100 SP Mirror 1 (left) 505.000000 SP Mirror 1 (right) 535.000000 SP Mirror 1 (stain) SP Mirror 2 (left) 600.000000 SP Mirror 2 (right) 700.000000 SP Mirror 2 (stain) SP Mirror 3 (left) 732.500000 SP Mirror 3 (right) 810.833333 SP Mirror 3 (stain) Objective HC PL APO 20.0x0.70 UV HC PL APO 20.0x0.70 UV Order number (Obj.) 506513 Numerical aperture (Obj.) 0.700000 SCANNER INFORMATION #2 RoiScan 0 IsSequential 0 ChaserUVShutter 0 ChaserVisibleShutter 0 MPShutter 0 UVShutter 0 VisibleShutter 1 ScanMode xyzt Active Pinhole [m] 0.000073 Pinhole [airy] 1.001570 Size-Width [µm] 750.000000 Size-Height [µm] 750.000000 Size-Depth [µm] -23.078802 StepSize 0.000001 Voxel-Width [µm] 1.464844 Voxel-Height [µm] 1.464844 Voxel-Depth [µm] 1.098991 Zoom 1.000000 Scan-Direction 1 Y-Scan-Direction 1 SequentialMode 0 Frame-Accumulation 1 Frame-Average 1 Line-Average 4 Resolution 8 Channels 2 Delay[min] [min] 3 Format-Width 512 Format-Height 512 Iterations 3 Sections 22 TIME INFORMATION #2 Stamped Dimension: 2 Stamp_0: 2010:11:25,17:05:55:401 Stamp_1: 2010:11:25,17:05:55:401 Stamp_2: 2010:11:25,17:06:01:901 Stamp_3: 2010:11:25,17:06:01:901 Stamp_4: 2010:11:25,17:06:08:401 Stamp_5: 2010:11:25,17:06:08:401 Stamp_6: 2010:11:25,17:06:14:916 Stamp_7: 2010:11:25,17:06:14:916 Stamp_8: 2010:11:25,17:06:21:416 Stamp_9: 2010:11:25,17:06:21:416 Stamp_10: 2010:11:25,17:06:27:932 Stamp_11: 2010:11:25,17:06:27:932 Stamp_12: 2010:11:25,17:06:34:447 Stamp_13: 2010:11:25,17:06:34:447 Stamp_14: 2010:11:25,17:06:40:947 Stamp_15: 2010:11:25,17:06:40:947 Stamp_16: 2010:11:25,17:06:47:462 Stamp_17: 2010:11:25,17:06:47:462 Stamp_18: 2010:11:25,17:06:53:978 Stamp_19: 2010:11:25,17:06:53:978 Stamp_20: 2010:11:25,17:07:00:478 Stamp_21: 2010:11:25,17:07:00:478 Stamp_22: 2010:11:25,17:07:06:993 Stamp_23: 2010:11:25,17:07:06:993 Stamp_24: 2010:11:25,17:07:13:493 Stamp_25: 2010:11:25,17:07:13:493 Stamp_26: 2010:11:25,17:07:20:09 Stamp_27: 2010:11:25,17:07:20:09 Stamp_28: 2010:11:25,17:07:26:524 Stamp_29: 2010:11:25,17:07:26:524 Stamp_30: 2010:11:25,17:07:33:24 Stamp_31: 2010:11:25,17:07:33:24 Stamp_32: 2010:11:25,17:07:39:540 Stamp_33: 2010:11:25,17:07:39:540 Stamp_34: 2010:11:25,17:07:46:55 Stamp_35: 2010:11:25,17:07:46:55 Stamp_36: 2010:11:25,17:07:52:555 Stamp_37: 2010:11:25,17:07:52:555 Stamp_38: 2010:11:25,17:07:59:71 Stamp_39: 2010:11:25,17:07:59:71 Stamp_40: 2010:11:25,17:08:05:571 Stamp_41: 2010:11:25,17:08:05:571 Stamp_42: 2010:11:25,17:08:12:86 Stamp_43: 2010:11:25,17:08:12:86 Stamp_44: 2010:11:25,17:08:55:383 Stamp_45: 2010:11:25,17:08:55:383 Stamp_46: 2010:11:25,17:09:01:898 Stamp_47: 2010:11:25,17:09:01:898 Stamp_48: 2010:11:25,17:09:08:414 Stamp_49: 2010:11:25,17:09:08:414 Stamp_50: 2010:11:25,17:09:14:914 Stamp_51: 2010:11:25,17:09:14:914 Stamp_52: 2010:11:25,17:09:21:429 Stamp_53: 2010:11:25,17:09:21:429 Stamp_54: 2010:11:25,17:09:27:929 Stamp_55: 2010:11:25,17:09:27:929 Stamp_56: 2010:11:25,17:09:34:445 Stamp_57: 2010:11:25,17:09:34:445 Stamp_58: 2010:11:25,17:09:40:960 Stamp_59: 2010:11:25,17:09:40:960 Stamp_60: 2010:11:25,17:09:47:460 Stamp_61: 2010:11:25,17:09:47:460 Stamp_62: 2010:11:25,17:09:53:976 Stamp_63: 2010:11:25,17:09:53:976 Stamp_64: 2010:11:25,17:10:00:491 Stamp_65: 2010:11:25,17:10:00:491 Stamp_66: 2010:11:25,17:10:06:991 Stamp_67: 2010:11:25,17:10:06:991 Stamp_68: 2010:11:25,17:10:13:507 Stamp_69: 2010:11:25,17:10:13:507 Stamp_70: 2010:11:25,17:10:20:06 Stamp_71: 2010:11:25,17:10:20:06 Stamp_72: 2010:11:25,17:10:26:522 Stamp_73: 2010:11:25,17:10:26:522 Stamp_74: 2010:11:25,17:10:33:38 Stamp_75: 2010:11:25,17:10:33:38 Stamp_76: 2010:11:25,17:10:39:537 Stamp_77: 2010:11:25,17:10:39:537 Stamp_78: 2010:11:25,17:10:46:53 Stamp_79: 2010:11:25,17:10:46:53 Stamp_80: 2010:11:25,17:10:52:569 Stamp_81: 2010:11:25,17:10:52:569 Stamp_82: 2010:11:25,17:10:59:68 Stamp_83: 2010:11:25,17:10:59:68 Stamp_84: 2010:11:25,17:11:05:584 Stamp_85: 2010:11:25,17:11:05:584 Stamp_86: 2010:11:25,17:11:12:84 Stamp_87: 2010:11:25,17:11:12:84 Stamp_88: 2010:11:25,17:11:55:380 Stamp_89: 2010:11:25,17:11:55:380 Stamp_90: 2010:11:25,17:12:01:896 Stamp_91: 2010:11:25,17:12:01:896 Stamp_92: 2010:11:25,17:12:08:396 Stamp_93: 2010:11:25,17:12:08:396 Stamp_94: 2010:11:25,17:12:14:911 Stamp_95: 2010:11:25,17:12:14:911 Stamp_96: 2010:11:25,17:12:21:427 Stamp_97: 2010:11:25,17:12:21:427 Stamp_98: 2010:11:25,17:12:27:927 Stamp_99: 2010:11:25,17:12:27:927 Stamp_100: 2010:11:25,17:12:34:442 Stamp_101: 2010:11:25,17:12:34:442 Stamp_102: 2010:11:25,17:12:40:958 Stamp_103: 2010:11:25,17:12:40:958 Stamp_104: 2010:11:25,17:12:47:458 Stamp_105: 2010:11:25,17:12:47:458 Stamp_106: 2010:11:25,17:12:53:973 Stamp_107: 2010:11:25,17:12:53:973 Stamp_108: 2010:11:25,17:13:00:473 Stamp_109: 2010:11:25,17:13:00:473 Stamp_110: 2010:11:25,17:13:06:989 Stamp_111: 2010:11:25,17:13:06:989 Stamp_112: 2010:11:25,17:13:13:504 Stamp_113: 2010:11:25,17:13:13:504 Stamp_114: 2010:11:25,17:13:20:04 Stamp_115: 2010:11:25,17:13:20:04 Stamp_116: 2010:11:25,17:13:26:520 Stamp_117: 2010:11:25,17:13:26:520 Stamp_118: 2010:11:25,17:13:33:35 Stamp_119: 2010:11:25,17:13:33:35 Stamp_120: 2010:11:25,17:13:39:535 Stamp_121: 2010:11:25,17:13:39:535 Stamp_122: 2010:11:25,17:13:46:51 Stamp_123: 2010:11:25,17:13:46:51 Stamp_124: 2010:11:25,17:13:52:551 Stamp_125: 2010:11:25,17:13:52:551 Stamp_126: 2010:11:25,17:13:59:66 Stamp_127: 2010:11:25,17:13:59:66 Stamp_128: 2010:11:25,17:14:05:582 Stamp_129: 2010:11:25,17:14:05:582 Stamp_130: 2010:11:25,17:14:12:82 Stamp_131: 2010:11:25,17:14:12:82 LUT DESCRIPTION #2 LUT_0 Name: Green Inverted (1=yes / 0=no): 0 LUT_1 Name: Red Inverted (1=yes / 0=no): 0 SEQUENTIAL INFORMATION #2 Sequence Count: 0 IMAGES INFORMATION #2 Number of Images: 132 Image Width: 512 Iamge Length: 512 Bits per Sample: 8 Samples per Pixel: 1 *************************************** NEXT IMAGE ********************************* DIMENSION DESCRIPTION #3 Pixel Size in Byte: 1 Resolution in Bit: 8 Max Value: 255.0000000000 Min Value: 0.000000e+000 Label: I Number of Dimensions: 4 Dimension_0: 120 Logical Size: 1024 Physical Length: 1.875000e-004 m Physical Origin: 0.000000e+000 m Dimension_1: 121 Logical Size: 1024 Physical Length: 1.875000e-004 m Physical Origin: 0.000000e+000 m Dimension_2: 6815843 Logical Size: 1 Physical Length: 0.000000e+000 Physical Origin: 0.000000e+000 Dimension_3: 122 Logical Size: 38 Physical Length: -2.560241e-005 m Physical Origin: 2.177629e-006 m Series Name: XYZ image 2 Description: HARDWARE PARAMETER #3 AOTF (405) 0.000000 AOTF (405) 0.000000 AOTF (458) 0.000000 AOTF (476) 0.000000 AOTF (488) 70.671551 AOTF (514) 0.000000 AOTF (543) 0.000000 AOTF (633) 0.000000 AOTF (458) 0.000000 AOTF (476) 0.000000 AOTF (488) 0.000000 AOTF (514) 0.000000 AOTF (543) 0.000000 AOTF (633) 0.000000 PMT 1 Active Active PMT 1 (Offs.) 3.600000 PMT 1 (HV) 483.333333 PMT 2 Inactive Inactive PMT 3 Inactive Inactive PMT Trans Inactive Inactive Beam Expander Beam Exp 6 Beam Exp 6 Excitation Beam Splitter FW RSP 500 RSP 500 UV Lens FW Lens 40xOil Lens 40xOil Hardware Type No. 2.000000 Scan Field Rotation -0.038943 Rotation Direction 1 X Scan Actuator Active Active X Scan Actuator (Gain) 2.000000 X Scan Actuator (Offs.) 0.000000 Y Scan Actuator Active Active Y Scan Actuator (Gain) 2.000000 Y Scan Actuator (Offs.) 0.000000 Z Scan Actuator Inactive Inactive Z Scan Actuator (POS) -0.000010 Scan Speed 400.000000 Phase 20.572917 Y-Phase 0.122100 SP Mirror 1 (left) 505.000000 SP Mirror 1 (right) 575.000000 SP Mirror 1 (stain) SP Mirror 2 (left) 600.000000 SP Mirror 2 (right) 700.000000 SP Mirror 2 (stain) SP Mirror 3 (left) 732.500000 SP Mirror 3 (right) 810.833333 SP Mirror 3 (stain) Objective HCX PL APO CS 40.0x1.25 OIL BD HCX PL APO CS 40.0x1.25 OIL BD UV Order number (Obj.) 506179 Numerical aperture (Obj.) 1.250000 SCANNER INFORMATION #3 RoiScan 0 IsSequential 0 ChaserUVShutter 0 ChaserVisibleShutter 0 MPShutter 0 UVShutter 0 VisibleShutter 1 ScanMode xyz Active Pinhole [m] 0.000082 Pinhole [airy] 1.002410 Size-Width [µm] 187.500000 Size-Height [µm] 187.500000 Size-Depth [µm] -25.602410 StepSize 0.000001 Voxel-Width [µm] 0.183105 Voxel-Height [µm] 0.183105 Voxel-Depth [µm] 0.691957 Zoom 2.000000 Scan-Direction 1 Y-Scan-Direction 1 SequentialMode 0 Frame-Accumulation 1 Frame-Average 1 Line-Average 4 Resolution 8 Channels 1 Format-Width 1024 Format-Height 1024 Sections 38 TIME INFORMATION #3 Stamped Dimension: 2 Stamp_0: 2010:11:25,17:24:47:295 Stamp_1: 2010:11:25,17:24:58:920 Stamp_2: 2010:11:25,17:25:10:545 Stamp_3: 2010:11:25,17:25:22:170 Stamp_4: 2010:11:25,17:25:33:795 Stamp_5: 2010:11:25,17:25:45:436 Stamp_6: 2010:11:25,17:25:57:60 Stamp_7: 2010:11:25,17:26:08:685 Stamp_8: 2010:11:25,17:26:20:326 Stamp_9: 2010:11:25,17:26:31:951 Stamp_10: 2010:11:25,17:26:43:576 Stamp_11: 2010:11:25,17:26:55:216 Stamp_12: 2010:11:25,17:27:06:841 Stamp_13: 2010:11:25,17:27:18:466 Stamp_14: 2010:11:25,17:27:30:91 Stamp_15: 2010:11:25,17:27:41:732 Stamp_16: 2010:11:25,17:27:53:357 Stamp_17: 2010:11:25,17:28:04:982 Stamp_18: 2010:11:25,17:28:16:622 Stamp_19: 2010:11:25,17:28:28:247 Stamp_20: 2010:11:25,17:28:39:872 Stamp_21: 2010:11:25,17:28:51:497 Stamp_22: 2010:11:25,17:29:03:137 Stamp_23: 2010:11:25,17:29:14:762 Stamp_24: 2010:11:25,17:29:26:387 Stamp_25: 2010:11:25,17:29:38:28 Stamp_26: 2010:11:25,17:29:49:653 Stamp_27: 2010:11:25,17:30:01:278 Stamp_28: 2010:11:25,17:30:12:903 Stamp_29: 2010:11:25,17:30:24:543 Stamp_30: 2010:11:25,17:30:36:168 Stamp_31: 2010:11:25,17:30:47:793 Stamp_32: 2010:11:25,17:30:59:434 Stamp_33: 2010:11:25,17:31:11:58 Stamp_34: 2010:11:25,17:31:22:683 Stamp_35: 2010:11:25,17:31:34:308 Stamp_36: 2010:11:25,17:31:45:949 Stamp_37: 2010:11:25,17:31:57:574 LUT DESCRIPTION #3 LUT_0 Name: Green Inverted (1=yes / 0=no): 0 SEQUENTIAL INFORMATION #3 Sequence Count: 0 IMAGES INFORMATION #3 Number of Images: 38 Image Width: 1024 Iamge Length: 1024 Bits per Sample: 8 Samples per Pixel: 1 *************************************** NEXT IMAGE ********************************* DIMENSION DESCRIPTION #4 Pixel Size in Byte: 1 Resolution in Bit: 8 Max Value: 255.0000000000 Min Value: 0.000000e+000 Label: I Number of Dimensions: 3 Dimension_0: 120 Logical Size: 1024 Physical Length: 3.750000e-004 m Physical Origin: 0.000000e+000 m Dimension_1: 121 Logical Size: 1024 Physical Length: 3.750000e-004 m Physical Origin: 0.000000e+000 m Dimension_2: 6815843 Logical Size: 1 Physical Length: 0.000000e+000 Physical Origin: 0.000000e+000 Series Name: Scale bar 100um Description: HARDWARE PARAMETER #4 AOTF (405) 0.000000 AOTF (405) 0.000000 AOTF (458) 0.000000 AOTF (476) 0.000000 AOTF (488) 24.004884 AOTF (514) 0.000000 AOTF (543) 0.000000 AOTF (633) 0.000000 AOTF (458) 0.000000 AOTF (476) 0.000000 AOTF (488) 0.000000 AOTF (514) 0.000000 AOTF (543) 0.000000 AOTF (633) 0.000000 PMT 1 Active Active PMT 1 (Offs.) 3.600000 PMT 1 (HV) 406.385836 PMT 2 Inactive Inactive PMT 3 Inactive Inactive PMT Trans Inactive Inactive Beam Expander Beam Exp 6 Beam Exp 6 Excitation Beam Splitter FW RT 30/70 RT 30/70 UV Lens FW Lens 10x Lens 10x Hardware Type No. 2.000000 Scan Field Rotation -0.038943 Rotation Direction 1 X Scan Actuator Active Active X Scan Actuator (Gain) 2.000000 X Scan Actuator (Offs.) 0.000000 Y Scan Actuator Active Active Y Scan Actuator (Gain) 2.000000 Y Scan Actuator (Offs.) 0.000000 Z Scan Actuator Inactive Inactive Z Scan Actuator (POS) -0.000014 Scan Speed 400.000000 Phase 20.572917 Y-Phase 0.122100 SP Mirror 1 (left) 485.000000 SP Mirror 1 (right) 495.000000 SP Mirror 1 (stain) SP Mirror 2 (left) 600.000000 SP Mirror 2 (right) 700.000000 SP Mirror 2 (stain) SP Mirror 3 (left) 732.500000 SP Mirror 3 (right) 810.833333 SP Mirror 3 (stain) Objective HC PL APO 20.0x0.70 UV HC PL APO 20.0x0.70 UV Order number (Obj.) 506513 Numerical aperture (Obj.) 0.700000 SCANNER INFORMATION #4 RoiScan 0 IsSequential 0 ChaserUVShutter 0 ChaserVisibleShutter 0 MPShutter 0 UVShutter 0 VisibleShutter 1 ScanMode xyz Active Pinhole [m] 0.000073 Pinhole [airy] 1.001570 Size-Width [µm] 375.000000 Size-Height [µm] 375.000000 Size-Depth [µm] -25.602410 StepSize 0.000001 Voxel-Width [µm] 0.366211 Voxel-Height [µm] 0.366211 Voxel-Depth [µm] 0.691957 Zoom 2.000000 Scan-Direction 1 Y-Scan-Direction 1 SequentialMode 0 Frame-Accumulation 1 Frame-Average 1 Line-Average 4 Resolution 8 Channels 1 Format-Width 1024 Format-Height 1024 Sections 38 TIME INFORMATION #4 Stamped Dimension: 2 Stamp_0: 2010:11:25,17:37:44:728 LUT DESCRIPTION #4 LUT_0 Name: Green Inverted (1=yes / 0=no): 0 SEQUENTIAL INFORMATION #4 Sequence Count: 0 IMAGES INFORMATION #4 Number of Images: 1 Image Width: 1024 Iamge Length: 1024 Bits per Sample: 8 Samples per Pixel: 1